Direct Dilution Vs Serial Dilution

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PMID: 6575015
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Serial dilutions are made by making the same dilution step over and over, using the previous dilution as the input to the next dilution in each step. Since the dilution-fold is the same in each step, the dilutions are a geometric series (constant ratio between any adjacent dilutions). Direct Improvement With Direct Dilution. Direct dilution vs serial dilution method The AZ researchers generated their range of compound concentrations using two different techniques. March 15, 2007 (Vol. 6) Many laboratory protocols require the serial dilution of reagents or compounds. IC50 assays, commonly used to evaluate drug efficacy, and assay development. Direct Dilution with the Echo Liquid Handler. Direct dilution is the process by which Echo Liquid Handlers generates the IC 50 curve, transferring precise droplets of solution directly to individual assay wells, without tips. Download Citation on ResearchGate SERIAL vs DIRECT DILUTION Time to apply new thinking to IC50 determination and dose-response analysis? The serial dilution method is standard practice in the.

Abstract

In a systematic approach, 37 duplicate samples of open system circuits (Bennett MA-1 ventilators) of patients in medical and surgical intensive care units were processed by direct and serial (APHA guidelines) dilutions. The paired difference test on 15 of the in-use circuitry solution samples indicated no difference between the direct and serial dilution methods (P less than 0.001). Seventy-seven additional respiratory therapy circuitry samples from similar intensive care patients were analyzed via a direct dilution method alone and processed microbiologically. The direct dilution procedure was a rapid and accurate means of evaluation of microbial contamination in the range of greater than or equal to 10 to less than or equal to 10(6) CFU/ml. High densities of organisms frequently were found. Sites of contamination included the proximal or patient end of the circuitry (heaviest), the nebulizer trap, and the distal or humidifier portions of the circuitry. The contaminants found were predominantly gram-negative nonfermenters: Acinetobacter calcoaceticus var. antitratus, Pseudomonas aeruginosa, Pseudomonas maltophilia, and Flavobacterium meningosepticum. Fermenters were Klebsiella pneumoniae, Proteus sp., Enterobacter cloacae, Citrobacter diversus, and Enterobacter agglomerans. Infrequently, gram-positive Streptococcus spp. and Staphylococcus spp. were noted.

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